12 rdalmatina2 O-Glycan rdalmatina2.jpg 588 611 9.413 13.821 No GalNAc-ol 1 neutral found in Rana palustris species (rpalustris2) Florea D., Maes E., Haddad M. and Strecker G. (2002), Biochimie, 84, 611-624 a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol 26649 (ID GlycomeDB 14992) 12 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 13 rdalmatina3 O-Glycan rdalmatina3.jpg 896 919 19.650 23.989 No GalNAc-ol 1 neutral HPLC peak N-8A Florea et al, 2002, Biochimie, 84, 611-624 a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33087) 13 spectrum_1D homonuclear Proton 1D 13 spectrum_90 homonuclear COSY90 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-GlcNAcp (1-4) (neutral) 11 rdalmatina1 O-Glycan rdalmatina1.jpg 531 554 9.835 14.231 Yes 1mg GalNAc-ol 1 neutral found also in Rana arvalis (rarvalis2), R. temporaria (rtemporaria31) and Bufo arenarum (barenarum1) species a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 80 (ID GlycomeDB 69) 11 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 14 rdalmatina4 O-Glycan rdalmatina4.jpg 839 862 20.338 24.624 No GalNAc-ol 1 neutral HPLC peak N-8B (mixture with rdalmatina3) Florea et al, 2002, Biochimie,84, 611-624 a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 14007 (ID GlycomeDB 8968) 14 spectrum_1D homonuclear Proton 1D 14 spectrum_90 homonuclear COSY90 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 15 rdalmatina7 O-Glycan rdalmatina7.jpg 773 796 14.762 19.119 No GalNAc-ol 1 acidic HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no referenced 15 spectrum_1D homonuclear Proton 1D 15 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) 16 rdalmatina8 O-Glycan rdalmatina8.jpg 854 873 13.819 18.214 No GalNAc-ol 2 acidic HSO3(4)-b-D-GalpNAc-(1-4)-b-D-3,6-an-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ no referenced 16 spectrum_1D homonuclear Proton 1D 16 spectrum_HMQCCarbon heteronuclear HMQCCarbon* 2D 16 spectrum_R1 homonuclear COSYR1 2D 16 spectrum_90 homonuclear COSY90 2D 16 spectrum_ROE homonuclear ROESY 2D 16 spectrum_R2 homonuclear COSYR2* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-GalNAcp (1-4) (acidic) with sulfate (4) [incr. 6] β-D-3,6-an-GlcNAcn (1-6) (neutral) 17 rdalmatina9 O-Glycan rdalmatina9.jpg 1000 1023 19.169 23.564 No GalNAc-ol 2 acidic HSO3(4)-b-D-GalpNAc-(1-4)-b-D-3,6-an-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 17 spectrum_1D homonuclear Proton 1D 17 spectrum_90 homonuclear COSY90 2D 17 spectrum_R1 homonuclear COSYR1 2D 17 spectrum_ROE homonuclear ROESY 2D 17 spectrum_R2 homonuclear COSYR2* 2D 17 spectrum_HMQCCarbon heteronuclear HMQCCarbon* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-GalNAcp (1-4) (acidic) with sulfate (4) [incr. 6] β-D-3,6-an-GlcNAcn (1-6) (neutral) 18 rdalmatina10 O-Glycan rdalmatina10.jpg 668 691 9.022 13.411 No GalNAc-ol 2 acidic sulfate-(O-6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ no referenced (sugabase ID : 5654, CCSD) 18 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 19 rdalmatina11 O-Glycan rdalmatina11.jpg 830 853 14.069 18.479 No GalNAc-ol 1 acidic HSO3(3)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 19 spectrum_1D homonuclear Proton 1D 19 spectrum_R2 homonuclear COSYR2 2D [incr. 3,4,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 20 rdalmatina12 O-Glycan rdalmatina12.jpg 814 837 14.402 18.795 Yes see barenarum10 GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 20 spectrum_1D homonuclear Proton 1D 20 spectrum_R2 homonuclear COSYR2 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 21 rdalmatina13 O-Glycan rdalmatina13.jpg 871 894 13.941 18.331 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)+ no referenced 21 spectrum_1D homonuclear Proton 1D 21 spectrum_90 homonuclear COSY90 2D 21 spectrum_R1 homonuclear COSYR1 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GlcNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 22 rdalmatina14 O-Glycan rdalmatina14.jpg 814 837 14.015 18.407 No GalNAc-ol 2 acidic HSO3(6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ no referenced 22 spectrum_1D homonuclear Proton 1D 22 spectrum_90 homonuclear COSY90 2D 22 spectrum_R1 homonuclear COSYR1 2D [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 23 rdalmatina15 O-Glycan rdalmatina15.jpg 960 983 19.393 23.785 No GalNAc-ol 2 acidic HSO3(6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 23 spectrum_1D homonuclear Proton 1D 23 spectrum_90 homonuclear COSY90 2D 23 spectrum_R1 homonuclear COSYR1 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 24 rdalmatina16 O-Glycan rdalmatina16.jpg 1017 1040 18.952 23.347 No GalNAc-ol 2 acidic HSO3(6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)+ 24 spectrum_1D homonuclear Proton* 1D [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GlcNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 25 rdalmatina17 O-Glycan rdalmatina17.jpg 879 902 11.073 15.453 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)-D-GalNAc-ol | a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)+ no referenced 25 spectrum_1D homonuclear Proton 1D 25 spectrum_90 homonuclear COSY90 2D 25 spectrum_R2 homonuclear COSYR2* 2D 25 spectrum_R1 homonuclear COSYR1* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GlcNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 26 rdalmatina18 O-Glycan rdalmatina18.jpg 976 999 19.276 23.645 No GalNAc-ol 2 acidic b-D-GlcpNAc-(1-6)+ | HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no referenced 26 spectrum_1D homonuclear Proton 1D 26 spectrum_90 homonuclear COSY90 2D 26 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 27 rdalmatina19 O-Glycan rdalmatina19.jpg 1179 1202 24.018 28.363 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-D-GlcpNAc-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 27 spectrum_1D homonuclear Proton 1D 27 spectrum_90 homonuclear COSY90 2D 27 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GlcNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 28 rdalmatina20 O-Glycan rdalmatina20.jpg 1179 1202 24.223 28.554 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 28 spectrum_1D homonuclear Proton 1D 28 spectrum_90 homonuclear COSY90 2D 28 spectrum_R1 homonuclear COSYR1 2D 28 spectrum_R2 homonuclear COSYR2* 2D 28 spectrum_ROE homonuclear ROESY 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-GlcNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 29 rdalmatina21 O-Glycan rdalmatina21.jpg 1122 1145 24.910 29.135 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-4)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 29 spectrum_1D homonuclear Proton 1D 29 spectrum_90 homonuclear COSY90 2D 29 spectrum_R1 homonuclear COSYR1 2D 29 spectrum_R2 homonuclear COSYR2* 2D 29 spectrum_ROE homonuclear ROESY 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 30 rdalmatina22 O-Glycan rdalmatina22.jpg 1064 1087 16.459 20.828 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no referenced 30 spectrum_1D homonuclear Proton 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 31 rdalmatina23 O-Glycan rdalmatina23.jpg 1121 1144 15.784 20.176 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | HSO3(3)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 31 spectrum_1D homonuclear Proton 1D 31 spectrum_90 homonuclear COSY90 2D 31 spectrum_ROE homonuclear ROESY* 2D 31 spectrum_R1 homonuclear COSYR1* 2D 31 spectrum_TOCSY homonuclear 2D [incr. 3,4,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 32 rdalmatina24 O-Glycan rdalmatina24.jpg 1655 1678 28.827 33.160 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ a-L-Fucp-(1-2)+ | | | HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-4)+ no referenced 32 spectrum_1D homonuclear Proton 1D 32 spectrum_90 homonuclear COSY90 2D 32 spectrum_ROE homonuclear ROESY* 2D 32 spectrum_R1 homonuclear COSYR1* 2D 32 spectrum_TOCSY homonuclear TOCSY 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 33 rdalmatina25 O-Glycan rdalmatina25.jpg 1655 1678 29.778 34.050 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ a-L-Fucp-(1-2)+ | | | HSO3(3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 33 spectrum_1D homonuclear Proton 1D 33 spectrum_R1 homonuclear COSYR1 2D 33 spectrum_R2 homonuclear COSYR2* 2D 33 spectrum_ROE homonuclear ROESY 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (acidic) with sulfate (3) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 34 rdalmatina26 O-Glycan rdalmatina26.jpg 1801 1824 34.050 39.039 No GalNAc-ol 2 acidic a-L-Fucp-(1-3)+ | HSO3(6)-b-D-GlcpNAc-(1-6)+ a-L-Fucp-(1-2)+ | | | HSO3(3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 34 spectrum_1D homonuclear Proton 1D 34 spectrum_HMQCCarbon heteronuclear HMQCCarbon* 2D 34 spectrum_ROE homonuclear ROESY* 2D 34 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (acidic) with sulfate (3) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 35 rdalmatina27 O-Glycan rdalmatina27.jpg 879 902 11.104 15.499 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)-D-GalNAc-ol | a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)+ no (ID GlycomeDB 33106) 35 spectrum_90 homonuclear COSY90* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 36 rdalmatina28 O-Glycan rdalmatina28.jpg 1130 1153 22.005 26.250 Yes 8.5 mg (purity ~90%) GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 36 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 37 rridibunda1 O-Glycan rridibunda1.jpg 814 837 14.015 18.407 Yes 12.8 mg GalNAc-ol 2 acidic 37 spectrum_1D homonuclear Proton 1D 37 spectrum_90 homonuclear COSY90* 2D [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 38 rridibunda2 O-Glycan rridibunda2.jpg 960 983 19.393 23.785 Yes 0.3 mg GalNAc-ol 2 acidic also founded in Rana dalmatina species 38 spectrum_1D homonuclear Proton 1D 38 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 39 rridibunda3 O-Glycan rridibunda3.jpg 773 796 14.762 19.119 Yes 3 mg GalNAc-ol 1 acidic also founded in Rana dalmatina species 39 spectrum_1D homonuclear Proton 1D 39 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) 40 rridibunda4 O-Glycan rridibunda4.jpg 937 960 18.590 22.978 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-3)+ | a-L-Fucp-(1-4)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ no (ID GlycomeDB 33108) 40 spectrum_1D homonuclear Proton 1D 40 spectrum_90 homonuclear COSY90 2D 40 spectrum_R2 homonuclear COSYR2* 2D [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] α-L-Fucp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 41 rridibunda5 O-Glycan rridibunda5.jpg 897 920 11.249 15.627 No GalNAc-ol 1 acidic no (ID GlycomeDB 33109) 41 spectrum_1D homonuclear Proton* 1D pseudoCad [incr. 3,3] α-D-NeuGcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-GalNAcp (1-4) (neutral) 42 rridibunda6 O-Glycan rridibunda6.jpg 1059 1082 16.381 20.763 No GalNAc-ol 1 acidic no (ID GlycomeDB 33110) 42 spectrum_1D homonuclear Proton 1D 42 spectrum_90 homonuclear COSY90* 2D [incr. 3,3] α-D-NeuGcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-GalNAcp (1-4) (neutral) [incr. 4,4,3] α-D-Galp (1-4) (neutral) 43 rridibunda7 O-Glycan rridibunda7.jpg 855 878 20.087 24.450 No GalNAc-ol 1 neutral 43 spectrum_1D homonuclear Proton 1D 43 spectrum_90 homonuclear COSY90 2D 43 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,3] β-D-Galp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 44 rridibunda8 O-Glycan rridibunda8.jpg 1058 1081 24.621 28.988 Yes 0.5mg GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | D-GalNAc-ol | b-D-Galp-(1-2)-a-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ 44 spectrum_1D homonuclear Proton 1D 44 spectrum_90 homonuclear COSY90 2D 44 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,3] β-D-Galp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 45 rridibunda9 O-Glycan rridibunda9.jpg 1138 1161 24.663 29.017 No GalNAc-ol 2 acidic 45 spectrum_1D homonuclear Proton 1D 45 spectrum_90 homonuclear COSY90* 2D [incr. 2,3,3] β-D-Galp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 46 rridibunda10 O-Glycan rridibunda10.jpg 1284 1307 see comments see comments No GalNAc-ol 2 acidic a-L-Fucp-(1-3)+ | HSO3(6)-b-D-GlcpNAc-(1-6)+ | b-D-Galp-(1-2)-a-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33114) 46 spectrum_1D homonuclear Proton 1D 46 spectrum_90 homonuclear COSY90* 2D [incr. 2,3,3] β-D-Galp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 47 rridibunda11 O-Glycan rridibunda11.jpg 1163 1186 25.794 30.096 No GalNAc-ol 1 neutral 47 spectrum_1D homonuclear Proton 1D 47 spectrum_90 homonuclear COSY90 2D 47 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,3,3] β-D-Galp (1-2) (neutral) [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 48 rridibunda12 O-Glycan rridibunda12.jpg 869 892 19.374 23.728 Yes 2.4 mg GalNAc-ol 1 acidic b-D-GlcpA-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33116) 48 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 49 rridibunda13 O-Glycan rridibunda13.jpg 1072 1095 23.909 28.254 No GalNAc-ol 2 acidic b-D-GlcpNAc-(1-6)+ | b-D-GlcpA-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33117) 49 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 50 rridibunda14 O-Glycan rridibunda14.jpg 1298 1321 28.976 33.340 No GalNAc-ol 2 acidic 50 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 51 rridibunda15 O-Glycan rridibunda15.jpg 1031 1054 24.287 28.650 No GalNAc-ol 1 acidic b-D-Galp-(1-4)+ | b-D-GlcAp-(1-3)-b-D-Galp-(1-3)- b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 51 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 52 rridibunda16 O-Glycan rridibunda16.jpg 1234 1257 28.879 33.234 No GalNAc-ol 2 acidic b-D-GlcpNAc-(1-6)+ b-D-Galp-(1-4)+ | | | b-D-GlcAp-(1-3)-b-D-Galp-(1-3)- b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 52 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 53 rridibunda17 O-Glycan rridibunda17.jpg 1314 1337 28.895 33.246 No GalNAc-ol 2 acidic [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 54 rridibunda18 O-Glycan rridibunda18.jpg 1460 1483 33.888 38.232 No GalNAc-ol 2 acidic [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 55 rridibunda19 O-Glycan rridibunda19.jpg 1234 1257 29.288 33.651 No GalNAc-ol 1 acidic [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) 56 rridibunda20 O-Glycan rridibunda20.jpg 1517 1540 33.896 38.257 No GalNAc-ol 2 acidic 56 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 57 rridibunda21 O-Glycan rridibunda21.jpg 1663 1686 38.904 43.255 No GalNAc-ol 2 acidic 57 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 58 rridibunda22 O-Glycan rridibunda22.jpg 1396 1419 33.811 38.176 No GalNAc-ol 1 acidic [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) 59 rridibunda23 O-Glycan rridibunda23.jpg 1679 1702 38.488 42.839 No GalNAc-ol 2 acidic 59 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 60 rridibunda24 O-Glycan rridibunda24.jpg 1825 1848 43.252 47.591 No GalNAc-ol 2 acidic 60 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,4,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 61 rridibunda25 O-Glycan rridibunda25.jpg 1572 1595 33.871 38.236 No GalNAc-ol 1 acidic 61 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3,4,4,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] α-D-GalNAcp (1-4) (neutral) 62 bviridis1 O-Glycan bviridis1.jpg 1083 1106 23.856 28.237 No GalNAc-ol 2 neutral a-L-Fucp-(1-4)- b-D-GlcpNAc-(1-6)+ | | b-D-GlcpNAc-(1-3)+ D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 62 spectrum_1D homonuclear Proton 1D 62 spectrum_90 homonuclear COSY90 2D 62 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] α-L-Fucp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 63 bviridis2 O-Glycan bviridis2.jpg 1042 1065 25.816 30.197 No GalNAc-ol 1 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 63 spectrum_1D homonuclear Proton 1D 63 spectrum_90 homonuclear COSY90 2D 63 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 64 bviridis3 O-Glycan bviridis3.jpg 1245 1268 28.626 33.027 No GalNAc-ol 2 neutral b-D-Galp-(1-4)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 64 spectrum_90 homonuclear COSY90 2D 64 spectrum_R1 homonuclear COSYR1 2D 64 spectrum_R2 homonuclear COSYR2* 2D 64 spectrum_HMQCCarbon heteronuclear HMQCCarbon 2D H group Lewis x [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,6] α-L-Fucp (1-3) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 65 bviridis4 O-Glycan bviridis4.jpg 1147 1170 29.231 33.231 No Gal-ol neutral b-D-Galp-(1-4)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-Gal-ol | a-L-Fucp-(1-2)+ no referenced 65 spectrum_1D homonuclear 1D 65 spectrum_90 homonuclear COSY90 2D 65 spectrum_R1 homonuclear COSYR1 2D 65 spectrum_R2 homonuclear COSYR2* 2D 65 spectrum_HSQCCarbon heteronuclear HMQCCarbon 2D H group Lewis x [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2] α-L-Fucp (1-2) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 66 bviridis5 O-Glycan bviridis5.jpg 1245 1268 29.429 33.662 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 66 spectrum_1D homonuclear Proton 1D 66 spectrum_90 homonuclear COSY90 2D 66 spectrum_R1 homonuclear COSYR1 2D 66 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 67 bviridis6 O-Glycan bviridis6.jpg 1391 1414 34.448 38.676 No GalNAc-ol 1 neutral a-L-Fucp-(1-4)+ | b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 67 spectrum_1D homonuclear Proton 1D 67 spectrum_R2 homonuclear COSYR2* 2D 67 spectrum_ROE homonuclear ROESY 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] α-L-Fucp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 68 bviridis7 O-Glycan bviridis7.jpg 1594 1617 38.976 43.199 No GalNAc-ol 2 neutral a-L-Fucp-(1-4)+ | b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)+ | b-D-GlcpNAc-(1-6)+ | | | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 68 spectrum_R2 homonuclear COSYR2* 2D 68 spectrum_ROE homonuclear ROESY* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] α-L-Fucp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 69 bviridis8 O-Glycan bviridis8.jpg 879 902 11.224 15.602 Yes see barenarum7 GalNAc-ol 1 acidic b-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ 12269 (ID GlycomeDB 7771) 69 spectrum_1D homonuclear Proton 1D 69 spectrum_90 homonuclear COSY90 2D 69 spectrum_R2 homonuclear COSYR2 2D 69 spectrum_HMQCCarbon heteronuclear HMQCCarbon 2D Cad [incr. 2,3] α-D-NeuAcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-GalNAcp (1-4) (neutral) 70 bviridis9 O-Glycan bviridis9.jpg 822 845 11.538 15.921 Yes see barenarum6 GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 9026 (ID GlycomeDB 6354) 70 spectrum_1D homonuclear Proton 1D 70 spectrum_90 homonuclear COSY90 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 71 bviridis10 O-Glycan bviridis10.jpg 676 699 6.165 10.543 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | D-GalNAc-ol | b-D-Galp-(1-3)+ 638 (ID GlycomeDB 512) 71 spectrum_1D homonuclear Proton 1D 71 spectrum_90 homonuclear COSY90 2D reduced Sialyl T [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 72 bviridis11 O-Glycan bviridis11.jpg 984 1007 16.378 20.704 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | D-GalNAc-ol | b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ 72 spectrum_1D homonuclear Proton 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 73 bviridis12 O-Glycan bviridis12.jpg 1130 1153 22.005 26.250 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 73 spectrum_1D homonuclear Proton 1D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 74 bviridis13 O-Glycan bviridis13.jpg 1333 1356 26.582 30.818 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | b-D-GlcpNAc-(1-6)+ D-GalNAc-ol | | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 74 spectrum_1D homonuclear Proton 1D 74 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 75 bviridis14 O-Glycan bviridis14.jpg 1641 1664 36.184 40.414 No GalNAc-ol 1 acidic HPLC peak NFI-23 Coppin et al, 2002, Carbohydr. Res., 337, 121-132 a-L-Fucp-(1-3)+ a-D-Neup5Ac-(2-6)+ | | b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ D-GalNAc-ol | | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 75 spectrum_R1 homonuclear COSYR1* 2D H group Lewis x [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 76 bviridis15 O-Glycan bviridis15.jpg 1787 1810 41.390 45.613 No GalNAc-ol 1 acidic a-L-Fucp-(1-3)+ a-D-Neup5Ac-(2-6)+ | | a-L-Fucp-(1-2)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ D-GalNAc-ol | | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 76 spectrum_R2 homonuclear COSYR2* 2D 76 spectrum_ROE homonuclear ROESY* 2D 76 spectrum_HMQCCarbon heteronuclear HMQCCarbon* 2D H group Lewis y [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 77 rarvalis1 O-Glycan rarvalis1.jpg 385 408 4.478 8.873 Yes see rtemporaria2 GalNAc-ol 1 neutral b-D-Galp-(1-3)-D-GalNAc-ol 375 (ID GlycomeDB : 316) 77 spectrum_1D homonuclear Proton* 1D T antigen [incr. 3] β-D-Galp (1-3) (neutral) 78 rarvalis2 O-Glycan rarvalis2.jpg 531 554 9.835 14.231 Yes 5.4 mg GalNAc-ol 1 neutral a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 80 (ID GlycomeDB 69) 78 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 79 rarvalis3 O-Glycan rarvalis3.jpg 588 611 9.001 13.395 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ 634 (ID GlycomeDB 508) 79 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 80 rarvalis4 O-Glycan rarvalis4.jpg 734 757 14.348 18.750 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 637 (ID GlycomeDB 511) H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 81 rarvalis5 O-Glycan rarvalis5.jpg 693 716 14.672 19.009 No GalNAc-ol 1 neutral b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33141) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 82 rarvalis6 O-Glycan rarvalis6.jpg 561 584 9.201 13.592 No GalNAc-ol 1 acidic b-D-GlcpA-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol no (ID GlycomeDB 33142) 82 spectrum_1D homonuclear Proton* 1D 82 spectrum_90 homonuclear COSY90 2D 82 spectrum_R1 homonuclear COSYR1 2D 82 spectrum_R2 homonuclear COSYR2 2D [incr. 3,3] β-D-GlcAp (1-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) 83 rarvalis7 O-Glycan rarvalis7.jpg 869 892 19.374 23.728 No GalNAc-ol 1 acidic b-D-GlcpA-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33116) 83 spectrum_1D homonuclear Proton 1D 83 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 84 rarvalis8 O-Glycan rarvalis8.jpg 773 796 14.760 19.122 No GalNAc-ol 1 acidic HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 84 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) 85 rarvalis9 O-Glycan rarvalis9.jpg 976 999 19.320 23.670 No GalNAc-ol 1 acidic b-D-GlcpNAc-(1-6)+ | HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 85 spectrum_1D homonuclear Proton 1D 85 spectrum_90 homonuclear COSY90 2D 85 spectrum_R1 homonuclear COSYR1 2D 85 spectrum_R2 homonuclear COSYR2* 2D 85 spectrum_ROE homonuclear ROESY 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 86 rarvalis10 O-Glycan rarvalis10.jpg 1072 1095 23.909 28.254 No GalNAc-ol 2 acidic b-D-GlcpNAc-(1-6)+ | b-D-GlcpA-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33117) 86 spectrum_1D homonuclear Proton 1D 86 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 87 rarvalis11 O-Glycan rarvalis11.jpg 976 999 19.276 23.645 No GalNAc-ol 2 acidic b-D-GlcpNAc-(1-6)+ | HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no referenced 87 spectrum_1D homonuclear Proton 1D 87 spectrum_R1 homonuclear COSYR1 2D 87 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 88 rarvalis12 O-Glycan rarvalis12.jpg 830 853 13.550 17.945 No GalNAc-ol 2 acidic HSO3(4)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol no 88 spectrum_1D homonuclear Proton 1D 88 spectrum_90 homonuclear COSY90 2D 88 spectrum_R1 homonuclear COSYR1 2D 88 spectrum_R2 homonuclear COSYR2* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (acidic) with sulfate (4) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 89 rarvalis13 O-Glycan rarvalis13.jpg 976 999 19.244 23.615 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ | b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 89 spectrum_1D homonuclear Proton* 1D 89 spectrum_90 homonuclear COSY90 2D 89 spectrum_R1 homonuclear COSYR1 1D 89 spectrum_R2 homonuclear COSYR2 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 90 rarvalis14 O-Glycan rarvalis14.jpg 1122 1145 24.910 29.135 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 90 spectrum_1D homonuclear Proton 1D 90 spectrum_90 homonuclear COSY90 2D 90 spectrum_R1 homonuclear COSYR1* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 91 rarvalis15 O-Glycan rarvalis15.jpg 976 999 18.963 23.355 No GalNAc-ol 2 acidic HSO3(6)+ | b-D-Galp-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol no 91 spectrum_1D homonuclear Proton 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 92 rarvalis16 O-Glycan rarvalis16.jpg 1179 1202 23.833 28.156 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ b-D-GlcpNAc-(1-6)+ | | | b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 92 spectrum_1D homonuclear Proton 1D 92 spectrum_90 homonuclear COSY90 2D 92 spectrum_R1 homonuclear COSYR1 2D 92 spectrum_R2 homonuclear COSYR2 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 93 rarvalis17 O-Glycan rarvalis17.jpg 1341 1364 28.339 32.662 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | | | b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 93 spectrum_1D homonuclear Proton 1D 93 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 94 rarvalis18 O-Glycan rarvalis18.jpg 1325 1348 29.508 33.732 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ b-D-GlcpNAc-(1-6)+ | | | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 94 spectrum_1D homonuclear Proton 1D 94 spectrum_90 homonuclear COSY90* 2D 94 spectrum_R1 homonuclear COSYR1 2D 94 spectrum_R2 homonuclear COSYR2 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 95 rarvalis19 O-Glycan rarvalis19.jpg 1487 1510 33.986 38.198 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | | | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 95 spectrum_1D homonuclear Proton 1D 95 spectrum_90 homonuclear COSY90* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 96 vgermanica1 O-Glycan vgermanica1.jpg 223 246 4.252 Yes see barenarum11 GalNAc-ol neutral for NMR spectrum see barenarum11 D-GalNAc-ol 1561 (ID GlycomeDB 1276) 97 vgermanica2 O-Glycan vgermanica2.jpg 330 353 4.263 No GalNAc-ol 1 zwitterionic 97 spectrum_HMQCPhosphorus heteronuclear HMQCPhosphorus* 2D 97 spectrum_HMQCCarbon heteronuclear HMQCCarbon* 2D 97 spectrum_1D homonuclear Proton* 1D 98 vgermanica3 O-Glycan vgermanica3.jpg 385 408 4.478 8.873 Yes see rtemporaria2 GalNAc-ol 1 neutral found in many animal species b-D-Galp-(1-3)-D-GalNAc-ol 375 (ID GlycomeDB : 316) T antigen [incr. 3] β-D-Galp (1-3) (neutral) 99 vgermanica4 O-Glycan vgermanica4.jpg 492 515 4.480 8.883 No GalNAc-ol 1 zwitterionic 99 spectrum_1D homonuclear Proton* 1D 99 spectrum_R1 homonuclear COSYR1* 2D [incr. 3] β-D-Galp (1-3) (neutral) 100 Man8Gna_664_2364_2234 N-Glycan Man8Gna_664_2364_2234.jpg 1517 1540 61.195 No a-D-GlcpNAc neutral It has 2 Linucs-ID number 17271 and 23309 a-D-Manp-(1-6)+ | a-D-Manp-(1-6)+ | | a-D-Manp-(1-2)-a-D-Manp-(1-3)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 17271 100 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,6,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) [incr. 6,6,4] α-D-Manp (1-6) (neutral) 101 Man8Gnb_664_2364_2234 N-Glycan Man8Gnb_664_2364_2234.jpg 1517 1540 61.195 No b-D-GlcpNAc neutral It has 2 Linucs-ID numbers 17271 and 23309 a-D-Manp-(1-6)+ | a-D-Manp-(1-6)+ | | a-D-Manp-(1-2)-a-D-Manp-(1-3)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 17271 101 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,6,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) [incr. 6,6,4] α-D-Manp (1-6) (neutral) 102 Man7Gna_2364_2234 N-Glycan Man7Gna_2364_2234.jpg 1355 1378 51.639 No a-D-GlcpNAc neutral a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 2140 102 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,6,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 103 Man7Gnb_2364_2234 N-Glycan Man7Gnb_2364_2234.jpg 1355 1378 51.639 No b-D-GlcpNAc neutral a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 2140 103 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,6,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 104 Man6Gna_364_2234 N-Glycan Man6Gna_364_2234.jpg 1193 1216 40.953 No a-D-GlcpNAc neutral a-D-Manp-(1-3)-a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 23253 104 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 105 Man6Gnb_364_2234 N-Glycan Man6Gnb_364_2234.jpg 1193 1216 40.953 No b-D-GlcpNAc neutral a-D-Manp-(1-3)-a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 23253 105 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 106 Man5Gna_64_2234 N-Glycan Man5Gna_64_2234.jpg 1031 1054 30.536 No a-D-GlcpNAc neutral a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 2845 106 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 107 Man5Gnb_64_2234 N-Glycan Man5Gnb_64_2234.jpg 1031 1054 30.536 No b-D-GlcpNAc neutral a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 2845 107 spectrum_1D homonuclear Proton* 1D [incr. 2,2,3,4] α-D-Manp (1-2) (neutral) [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 108 Man4Gna_64_234 N-Glycan Man4Gna_64_234.jpg 869 892 25.250 No a-D-GlcpNAc neutral a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 25658 108 spectrum_1D homonuclear Proton* 1D [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 109 Man4Gnb_64_234 N-Glycan Man4Gnb_64_234.jpg 869 892 25.250 No b-D-GlcpNAc neutral a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-2)-a-D-Manp-(1-3)+ 25658 109 spectrum_1D homonuclear Proton* 1D [incr. 2,3,4] α-D-Manp (1-2) (neutral) [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 110 Man4Gna_364_34 N-Glycan Man4Gna_364_34.jpg 869 892 30.184 No a-D-GlcpNAc neutral a-D-Manp-(1-3)-a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-3)+ 25540 110 spectrum_1D homonuclear Proton* 1D [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 111 Man4Gnb_364_34 N-Glycan Man4Gnb_364_34.jpg 869 892 30.184 No b-D-GlcpNAc neutral a-D-Manp-(1-3)-a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-3)+ 25540 111 spectrum_1D homonuclear Proton* 1D [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 3,6,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 112 Man3Gna_64_34 N-Glycan Man3Gna_64_34.jpg 707 730 19.958 No a-D-GlcpNAc neutral a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-a-D-GlcpNAc | a-D-Manp-(1-3)+ 8457 112 spectrum_1D homonuclear Proton* 1D [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 113 Man3Gnb_64_34 N-Glycan Man3Gnb_64_34.jpg 707 730 19.958 No b-D-GlcpNAc neutral a-D-Manp-(1-6)+ | b-D-Manp-(1-4)-b-D-GlcpNAc | a-D-Manp-(1-3)+ 8457 113 spectrum_1D homonuclear Proton* 1D [incr. 3,4] α-D-Manp (1-3) (neutral) [incr. 4] β-D-Manp (1-4) (neutral) [incr. 6,4] α-D-Manp (1-6) (neutral) 114 rtemporaria1 O-Glycan rtemporaria1.jpg 223 246 4.252 GalNAc-ol neutral D-GalNAc-ol 1561 (ID GlycomeDB 1276) 115 rtemporaria2 O-Glycan rtemporaria2.jpg 385 408 4.478 8.873 Yes 5mg GalNAc-ol 1 neutral b-D-Galp-(1-3)-D-GalNAc-ol 375 (ID GlycomeDB : 316) T antigen [incr. 3] β-D-Galp (1-3) (neutral) 116 rtemporaria10 O-Glycan rtemporaria10.jpg 773 796 14.594 18.981 Yes 1mg GalNAc-ol 1 acidic HSO3-(3)- b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fuc-(1-2)+ 116 spectrum_R2 homonuclear COSYR2* 2D 116 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (acidic) with sulfate (3) 117 rtemporaria11 O-Glycan rtemporaria11.jpg 935 958 19.673 24.032 Yes 1mg GalNAc-ol 1 acidic b-D-Galp-(1-3)+ | HSO3-(3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fuc-(1-2)+ no 117 spectrum_R2 homonuclear COSYR2* 2D 117 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (acidic) with sulfate (3) 118 rtemporaria3 O-Glycan rtemporaria3.jpg 385 408 5.170 9.543 Yes GalNAc-ol 8 neutral a-D-Galp-(1-3)-D-GalNAc-ol 7829 118 spectrum_90 homonuclear COSY90* 2D [incr. 3] α-D-Galp (1-3) (neutral) 119 rtemporaria5 O-Glycan rtemporaria5.jpg 635 658 6.811 11.176 Yes 1mg GalNAc-ol 8 acidic a-D-Kdnp-(2-6)+ | a-D-Galp-(1-3)-D-GalNAc-ol no 119 spectrum_1D homonuclear Proton* 1D [incr. 3] α-D-Galp (1-3) (neutral) [incr. 6] α-D-Kdnp (2-6) (acidic) 120 rtemporaria6 O-Glycan rtemporaria6.jpg 635 658 6.124 10.501 Yes 10.2mg (see comments) GalNAc-ol 1 acidic a-D-Kdnp-(2-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol no 120 spectrum_R2 homonuclear COSYR2* 2D 120 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-Kdnp (2-6) (acidic) 121 rtemporaria7 O-Glycan rtemporaria7.jpg 781 802 12.105 16.526 Yes GalNAc-ol 1 acidic a-D-Kdnp-(2-6)+ | a-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 121 spectrum_R2 homonuclear COSYR2* 2D 121 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] α-D-Galp (1-3) (neutral) [incr. 6] α-D-Kdnp (2-6) (acidic) 122 rtemporaria8 O-Glycan rtemporaria8.jpg 465 488 4.592 8.988 Yes GalNAc-ol 1 acidic HSO3(3)-b-D-Galp-(1-3)-D-GalNAc-ol no 122 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (acidic) with sulfate (3) 123 rtemporaria9 O-Glycan rtemporaria9.jpg 627 650 9.203 13.592 Yes 23.4mg GalNAc-ol 1 acidic HSO3(3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no 123 spectrum_R2 homonuclear COSYR2* 2D 123 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (acidic) with sulfate (3) 124 rtemporaria4 O-Glycan rtemporaria4.jpg 473 496 1.660 5.903 Yes 1.2mg (see comments) GalNAc-ol acidic a-D-Kdnp-(2-6)-D-GalNAc-ol no [incr. 6] α-D-Kdnp (2-6) (acidic) 125 rtemporaria12 O-Glycan rtemporaria12.jpg 1081 1104 25.156 29.476 Yes GalNAc-ol 1 acidic a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ | HSO3(3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 125 spectrum_R2 homonuclear COSYR2* 2D 125 spectrum_HMQC heteronuclear HMQCCarbon* 1D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (acidic) with sulfate (3) 126 rtemporaria13 O-Glycan rtemporaria13.jpg 1015 1038 24.770 29.114 Yes GalNAc-ol 1 acidic a-L-Fucp-(1-2)-b-D-GlcAp-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 126 spectrum_1D homonuclear Proton* 1D 126 spectrum_R2 homonuclear COSYR2* 1D 126 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 127 rtemporaria14 O-Glycan rtemporaria14.jpg 1015 1038 24.557 28.954 Yes GalNAc-ol 1 acidic a-L-Fucp-(1-2)-b-D-GlcAp-(1-3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 127 spectrum_1D homonuclear Proton* 1D 127 spectrum_R2 homonuclear COSYR2* 2D 127 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] β-D-GlcAp (1-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-3) (neutral) 128 rtemporaria15 O-Glycan rtemporaria15.jpg 668 691 9.022 13.411 Yes GalNAc-ol 2 acidic HSO3(6)+ | b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ 128 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 129 rtemporaria16 O-Glycan rtemporaria16.jpg 814 837 14.402 18.795 Yes 0.1 mg and see barenarum10 GalNAc-ol 2 acidic HSO3(6)+ | b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 129 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 130 rtemporaria17 O-Glycan rtemporaria17.jpg 1122 1145 24.233 28.568 Yes GalNAc-ol 2 acidic HSO3(6)b-D-GlcpNAc-(1-6)+ | | a-L-Fucp-(1-3)+ | D-GalNAc-ol | b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no 130 spectrum_R2 homonuclear COSYR2* 2D 130 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 131 rtemporaria18 O-Glycan rtemporaria18.jpg 1444 1467 34.326 38.668 Yes 7.7mg GalNAc-ol 2 acidic HSO3(6) + | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-GlcAp-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 131 spectrum_R2 homonuclear COSYR2* 2D 131 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 132 rtemporaria19 O-Glycan rtemporaria19.jpg 715 738 6.242 10.625 Yes 1.3mg GalNAc-ol 1 acidic a-D-Kdnp-(2-6)+ | HSO3(3)-a-D-Galp-(1-3)-D-GalNAc-ol no 132 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 6] α-D-Kdnp (2-6) (acidic) 133 rtemporaria20 O-Glycan rtemporaria20.jpg 1202 1225 24.435 28.783 Yes GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)+ | | a-L-Fucp-(1-3)+ | D-GalNAc-ol | HSO3(3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no 133 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (acidic) with sulfate (3) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 134 rtemporaria21 O-Glycan rtemporaria21.jpg 803 826 13.897 18.284 Yes GalNAc-ol 1 acidic HSO3(3)-b-D-GlcAp-(1-3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no 134 spectrum_1D homonuclear Proton* 1D part of HNK-1 [incr. 3,4,3] β-D-GlcAp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 136 rtemporaria23 O-Glycan rtemporaria23.jpg 1111 1134 24.130 28.518 No GalNAc-ol 1 acidic HSO3(3)-b-D-GlcAp-(1-3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | | b-D-Galp-(1-4)+ a-L-Fucp-(1-2)+ no 136 spectrum_R2 homonuclear COSYR2* 2D part of HNK-1 [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] β-D-GlcAp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] β-D-Galp (1-4) (neutral) 135 rtemporaria22 O-Glycan rtemporaria22.jpg 949 972 19.286 23.675 No GalNAc-ol 1 acidic HSO3(3)-b-D-GlcAp-(1-3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 135 spectrum_R2 homonuclear COSYR2* 2D part of HNK-1 [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] β-D-GlcAp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 137 rtemporaria24 O-Glycan rtemporaria24.jpg 1111 1134 24.421 28.753 No GalNAc-ol 1 acidic b-D-Galp-(1-4)+ | HSO 3(3)-b-D-GlcAp-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 137 spectrum_R2 homonuclear COSYR2* 2D 137 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] β-D-GlcAp (1-3) (acidic) with sulfate (3) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 138 rtemporaria25 O-Glycan rtemporaria25.jpg 1257 1280 29.592 33.924 No GalNAc-ol 1 acidic a-L-Fucp-(1-2)-b-D-Galp-(1-4)+ | HSO3(3)-b-D-GlcAp-(1-3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 138 spectrum_R2 homonuclear COSYR2* 2D 138 spectrum_ROESY homonuclear ROESY* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] β-D-GlcAp (1-3) (acidic) with sulfate (3) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 4,4,3] β-D-Galp (1-4) (neutral) 139 rtemporaria26 O-Glycan rtemporaria26.jpg 531 554 10.429 14.854 Yes GalNAc-ol 8 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-D-GalNAc-ol no 139 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] α-D-Galp (1-3) (neutral) 140 rtemporaria27 O-Glycan rtemporaria27.jpg 693 716 14.485 18.878 Yes GalNAc-ol 1 neutral b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 140 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 141 rtemporaria28 O-Glycan rtemporaria28.jpg 693 716 14.672 19.009 Yes GalNAc-ol 1 neutral b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no (ID GlycomeDB 33141) 141 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 142 rtemporaria29 O-Glycan rtemporaria29.jpg 893 919 18.994 23.393 Yes Gal-ol 2 neutral b-D-GlcpNAc-(1-6)+ | b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 142 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 143 rtemporaria33 O-Glycan rtemporaria33.jpg 734 757 14.348 18.750 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 637 (ID GlycomeDB 511) H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 144 rtemporaria30 O-Glycan rtemporaria30.jpg 855 878 19.595 23.933 Yes 3.7mg GalNAc-ol 1 neutral b-D-Galp-(1-4)+ | b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 144 spectrum_R2 homonuclear COSYR2* 2D 144 spectrum_ROESY homonuclear ROESY* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 145 rtemporaria31 O-Glycan rtemporaria31.jpg 531 554 9.835 14.231 Yes GalNAc-ol 1 neutral a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 80 (ID GlycomeDB 69) H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 146 rtemporaria32 O-Glycan rtemporaria32.jpg 893 919 9.094 13.483 Yes GalNAc-ol 1 neutral b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol 8186 146 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 147 rtemporaria34 O-Glycan rtemporaria34.jpg 839 862 20.010 24.388 Yes GalNAc-ol 1 neutral b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 147 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 148 rtemporaria35 O-Glycan rtemporaria35.jpg 791 814 13.539 17.929 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ 26984 (ID GlycomeDB 15239) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 149 rtemporaria36 O-Glycan rtemporaria36.jpg 937 960 18.879 23.277 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no 149 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 150 rtemporaria37 O-Glycan rtemporaria37.jpg 1147 1170 30.522 34.908 Yes GalNAc-ol 1 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-b-D-Galp-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ 150 spectrum_R1 homonuclear COSYR1* 2D 150 spectrum_ROESY homonuclear ROESY* 2D [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-Galp (1-4) (neutral) 152 rclamitans2 O-Glycan rclamitans2.jpg 734 757 13.993 18.386 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ no 152 spectrum_1D homonuclear Proton* 1D 152 spectrum_90 homonuclear COSY90* 2D [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 151 rclamitans1 O-Glycan rclamitans1.jpg 588 611 9.001 13.395 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ 634 (ID GlycomeDB 508) 151 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 153 rclamitans3 O-Glycan rclamitans3.jpg 1099 1122 23.238 27.623 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-Galp-(1-3)+ no 153 spectrum_1D homonuclear Proton 1D 153 spectrum_90 homonuclear COSY90* 2D [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 154 rclamitans4 O-Glycan rclamitans4.jpg 1245 1268 28.242 32.628 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-Galp-(1-3)+ no 154 spectrum_1D homonuclear Proton 1D 154 spectrum_90 homonuclear COSY90* 2D 154 spectrum_R1 homonuclear COSYR1 2D 154 spectrum_R2 homonuclear COSYR2 2D 154 spectrum_ROE homonuclear ROESY 2D 154 spectrum_HMQCCarbon heteronuclear HMQCCarbon 2D [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 155 rclamitans5 O-Glycan rclamitans5.jpg 1407 1430 32.816 37.264 Yes GalNAc-ol 2 neutral b-D-Galp-(1-4)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-Galp-(1-3)+ no 155 spectrum_1D homonuclear Proton 1D 155 spectrum_R2 homonuclear COSYR2* 2D 155 spectrum_R1 homonuclear COSYR1 2D 155 spectrum_90 homonuclear COSY90 2D 155 spectrum_ROE homonuclear ROESY 2D Lewis x [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 156 rclamitans6 O-Glycan rclamitans6.jpg 896 919 18.737 23.121 Yes GalNAc-ol 1 neutral a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-Galp-(1-3)+ no 156 spectrum_1D homonuclear Proton* 1D [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 157 rclamitans7 O-Glycan rclamitans7.jpg 1058 1081 23.298 27.680 Yes GalNAc-ol 1 neutral b-D-Galp-(1-4)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-Galp-(1-3)+ no 157 spectrum_1D homonuclear Proton 1D 157 spectrum_R2 homonuclear COSYR2* 2D Lewis x [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 158 rclamitans8 O-Glycan rclamitans8.jpg 937 960 18.570 22.969 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)+ | b-D-GalpNAc-(1-3)-b-D-Galp-(1-3)- D-GalNAc-ol no 158 spectrum_1D homonuclear Proton 1D 158 spectrum_90 homonuclear COSY90 2D 158 spectrum_R1 homonuclear COSYR1* 2D [incr. 3,3] β-D-GalNAcp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 159 rclamitans9 O-Glycan rclamitans9.jpg 1099 1122 23.161 27.622 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-3)+ | b-D-GalpNAc-(1-3)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | b-D-Galp-(1-3)- D-GalNAc-ol no 159 spectrum_R2 homonuclear COSYR2* 2D Internal Lewis x [incr. 3,4,6] β-D-GalNAcp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 160 rclamitans10 O-Glycan rclamitans10.jpg 1302 1325 27.727 32.339 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-3)+ | b-D-GalpNAc-(1-3)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | b-D-GalpNAc-(1-3)-b-D-Galp-(1-3)- D-GalNAc-ol no 160 spectrum_1D homonuclear Proton 1D 160 spectrum_90 homonuclear COSY90 2D 160 spectrum_R1 homonuclear COSYR1* 2D 160 spectrum_R2 homonuclear COSYR2 2D 160 spectrum_ROE homonuclear ROESY 2D Internal Lewis x [incr. 3,3] β-D-GalNAcp (1-3) (neutral) [incr. 3,4,6] β-D-GalNAcp (1-3) (neutral) [incr. 3,6] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 161 rclamitans11 O-Glycan rclamitans11.jpg 1099 1122 23.476 27.859 Yes GalNAc-ol 1 neutral a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-GalpNAc-(1-3)-b-D-Galp-(1-3)+ no 161 spectrum_1D homonuclear Proton 1D 161 spectrum_90 homonuclear COSY90 2D 161 spectrum_R1 homonuclear COSYR1* 2D 161 spectrum_R2 homonuclear COSYR2 2D [incr. 3,3,3] β-D-GlcNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 162 rclamitans12 O-Glycan rclamitans12.jpg 1302 1325 27.825 32.212 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-6)-b-D-Galp-(1-3)- D-GalNAc-ol | b-D-GalpNAc-(1-3)-b-D-Galp-(1-3)+ no 162 spectrum_1D homonuclear Proton 1D 162 spectrum_90 homonuclear COSY90 2D 162 spectrum_R1 homonuclear COSYR1 2D 162 spectrum_R2 homonuclear COSYR2* 2D [incr. 3,3,3] β-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,6,3] α-L-Fucp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 163 rclamitans13 O-Glycan rclamitans13.jpg 856 879 19.956 24.346 Yes GalNAc-ol 1 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) 164 rclamitans14 O-Glycan rclamitans14.jpg 1059 1082 24.493 28.883 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no 164 spectrum_1D homonuclear Proton 1D 164 spectrum_90 homonuclear COSY90 2D 164 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 165 rclamitans15 O-Glycan rclamitans15.jpg 1367 1390 34.924 38.699 Yes GalNAc-ol neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | D-GalNAc-ol | a-L-Fucp-(1-2)-a-D-Galp-(1-3)+ no 165 spectrum_1D homonuclear Proton 1D 165 spectrum_90 homonuclear COSY90 2D 165 spectrum_R1 homonuclear COSYR1* 2D 165 spectrum_R2 homonuclear COSYR2 2D H group [incr. 2,3,4,4,6] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 166 rclamitans16 O-Glycan rclamitans16.jpg 1399 1422 33.954 38.488 Yes GalNAc-ol 2 neutral a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no 166 spectrum_1D homonuclear Proton 1D 166 spectrum_90 homonuclear COSY90* 2D 166 spectrum_R1 homonuclear COSYR1 2D 166 spectrum_R2 homonuclear COSYR2 2D [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 167 rclamitans17 O-Glycan rclamitans17.jpg 1383 1406 33.979 38.369 No GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no 167 spectrum_1D homonuclear Proton 1D 167 spectrum_90 homonuclear COSY90 2D [incr. 2,3,4,4,6] α-L-Fucp (1-2) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 168 rclamitans18 O-Glycan rclamitans18.jpg 1383 1406 33.987 38.377 No GalNAc-ol 2 neutral a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no 168 spectrum_1D homonuclear Proton 1D 168 spectrum_90 homonuclear COSY90 2D [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 169 rclamitans19 O-Glycan rclamitans19.jpg 1545 1568 39.188 43.577 Yes GalNAc-ol 2 neutral a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no 169 spectrum_1D homonuclear Proton 1D 169 spectrum_90 homonuclear COSY90 2D 169 spectrum_R1 homonuclear COSYR1* 1D [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 170 rclamitans20 O-Glycan rclamitans20.jpg 1691 1714 44.434 48.821 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-D-GalNAc-ol no 170 spectrum_1D homonuclear Proton 1D 170 spectrum_90 homonuclear COSY90* 2D 170 spectrum_HMQC heteronuclear HMQCCarbon* 2D 170 spectrum_HMQC heteronuclear HMQCCarbon* 2D [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 171 rclamitans21 O-Glycan rclamitans21.jpg 1853 1875 49.237 53.775 Yes GalNAc-ol 1 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol no 171 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6,3] α-D-Galp (1-4) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 172 rclamitans22 O-Glycan rclamitans22.jpg 2000 2023 49.375 53.959 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-b-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 172 spectrum_1D homonuclear Proton 1D 172 spectrum_90 homonuclear COSY90* 2D [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 173 rclamitans23 O-Glycan rclamitans23.jpg 2057 2080 53.744 58.132 No GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | b-D-GlcpNAc-(1-6)+ no 173 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6,3] α-D-Galp (1-4) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 174 rclamitans24 O-Glycan rclamitans24.jpg 2219 2240 58.143 62.529 Yes 1mg GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gal-(1-4)-b-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ no 174 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6,3] α-D-Galp (1-4) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 175 rclamitans25 O-Glycan rclamitans25.jpg 2543 2566 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Gapl-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ no [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 177 rclamitans27 O-Glycan rclamitans27.jpg 2689 2712 68.455 72.841 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ no 177 spectrum_1D homonuclear Proton 1D 177 spectrum_90 homonuclear COSY90 2D 177 spectrum_R1 homonuclear COSYR1* 2D 177 spectrum_HMQC heteronuclear HMQCCarbon 2D [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 176 rclamitans26 O-Glycan rclamitans26.jpg 2689 2712 68.452 72.840 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)-a-D-Galp-(1-3)-a-D-Galp-(1-4)-b-Galp-(1-4)-b-D-GlcpNAc-(1-6)+ no [incr. 2,3,4,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 2,3,4,4,6] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3,4,4,6] α-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6,3] α-D-Galp (1-4) (neutral) [incr. 4,4,6] α-D-Galp (1-4) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 178 xtropicalis1 O-Glycan xtropicalis1.jpg 588 611 9.001 13.395 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ 634 (ID GlycomeDB 508) 178 spectrum_1D homonuclear Proton* 1D 178 spectrum_90 homonuclear COSY90* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 179 xtropicalis2 O-Glycan xtropicalis2.jpg 734 757 14.348 18.750 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 637 (ID GlycomeDB 511) 179 spectrum_1D homonuclear Proton* 1D 179 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 180 xtropicalis3 O-Glycan xtropicalis3.jpg 734 757 15.289 19.590 Yes GalNAc-ol 1 neutral a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 639 (ID GlycomeDB 513) 180 spectrum_1D homonuclear Proton* 1D 180 spectrum_90 homonuclear COSY90* 1D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 181 xtropicalis4 O-Glycan xtropicalis4.jpg 734 757 14.145 18.423 Yes GalNAc-ol 3 neutral b-D-Galp-(1-3)-b-D-GlcpNAc-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-4)+ 181 spectrum_1D homonuclear Proton* 1D 181 spectrum_90 homonuclear COSY90* 2D Lewis a [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-GlcNAcp (1-3) (neutral) [incr. 4,3] α-L-Fucp (1-4) (neutral) 182 xtropicalis5 O-Glycan xtropicalis5.jpg 896 919 20.431 24.731 Yes GalNAc-ol neutral a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 182 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 183 xtropicalis6 O-Glycan xtropicalis6.jpg 937 960 19.833 24.133 Yes 0.1 mg and see barenarum12 GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 1674 (ID 1384 in GlycomeDB) 183 spectrum_1D homonuclear Proton* 1D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 184 xtropicalis7 O-Glycan xtropicalis7.jpg 896 919 18.511 22.902 Yes GalNAc-ol 2 neutral a-L-Fucp-(1-4)+ | b-D-Galp-(1-3)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ no 184 spectrum_1D homonuclear Proton* 1D 184 spectrum_90 homonuclear COSY90* 1D Lewis a [incr. 3,6] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] α-L-Fucp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 185 xtropicalis8 O-Glycan xtropicalis8.jpg 1099 1122 24.967 29.270 Yes GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 185 spectrum_1D homonuclear Proton* 1D 185 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 186 xtropicalis9 O-Glycan xtropicalis9.jpg 1017 1040 19.863 24.160 Yes GalNAc-ol 2 acidic 6S-b-D-GlcpNAc-(1-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 186 spectrum_1D homonuclear Proton* 1D 186 spectrum_R1 homonuclear COSYR1* 2D 186 spectrum_90 homonuclear COSY90 2D 186 spectrum_R2 homonuclear COSYR2 1D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 187 xtropicalis10 O-Glycan xtropicalis10.jpg 676 699 6.165 10.543 Yes GalNAc-ol 1 acidic a-D-NeupNAc-(2-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol 638 (ID GlycomeDB 512) 187 spectrum_1D homonuclear Proton* 1D 187 spectrum_R1 homonuclear COSYR1* 2D 187 spectrum_90 homonuclear COSY90 2D 187 spectrum_R2 homonuclear COSYR2 2D Sialyl T [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 188 xtropicalis11 O-Glycan xtropicalis11.jpg 1025 1048 15.846 20.099 Yes GalNAc-ol 3 acidic a-D-NeupNAc-(2-6)+ | b-D-Galp-(1-3)-b-D-GlcpNAc-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-4)+ no 188 spectrum_1D homonuclear Proton* 1D 188 spectrum_90 homonuclear COSY90* 2D Lewis a [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-GlcNAcp (1-3) (neutral) [incr. 4,3] α-L-Fucp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 189 xtropicalis12 O-Glycan xtropicalis12.jpg 1187 1210 22.126 26.422 Yes GalNAc-ol 1 acidic a-D-NeupNAc-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 189 spectrum_1D homonuclear Proton* 1D 189 spectrum_90 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 190 rpalustris1 O-Glycan rpalustris1.jpg 588 611 9.392 13.799 Yes 0.7mg GalNAc-ol 1 neutral HLPC peak N-1 Ref : Maes E. et al Eur. J. Biochem, 1999, 264, 301-313 a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol 376 (ID GlycomeDB 317) 190 spectrum_1D homonuclear Proton* 1D 190 spectrum_R2 homonuclear COSYR2* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GlcNAcp (1-4) (neutral) 191 rpalustris2 O-Glycan rpalustris2.jpg 588 611 9.413 13.821 No GalNAc-ol 1 neutral a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol 26649 (ID GlycomeDB 14992) 191 spectrum_R2 homonuclear COSYR2* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 192 rpalustris3 O-Glycan rpalustris3.jpg 588 611 9.001 13.395 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)+ 634 (ID GlycomeDB 508) 192 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 193 rpalustris4 O-Glycan rpalustris4.jpg 734 757 14.348 18.750 Yes 0.9 mg GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ 637 (ID GlycomeDB 511) 193 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 194 rpalustris5 O-Glycan rpalustris5.jpg 750 773 13.953 18.362 GalNAc-ol 1 neutral b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no (ID GlycomeDB 34485) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 195 rpalustris6 O-Glycan rpalustris6.jpg 879 902 11.104 15.499 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no (ID GlycomeDB 33106) 195 spectrum_1D homonuclear Proton* 1D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 196 rpalustris7 O-Glycan rpalustris7.jpg 1033 1056 18.548 22.927 No GalNAc-ol 2 acidic HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)+ no referenced 196 spectrum_R2 homonuclear COSYR2* 2D [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 197 rpalustris8 O-Glycan rpalustris8.jpg 1187 1210 21.057 25.456 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)+ no referenced 197 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 198 rpalustris9 O-Glycan rpalustris9.jpg 1187 1210 21.208 25.607 No GalNAc-ol 1 acidic a-L-Fucp-(1-2)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ no referenced 198 spectrum_R2 homonuclear COSYR2* 2D H group [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-NeuAcp (2-3) (acidic) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 199 rpalustris10 O-Glycan rpalustris10.jpg 514 537 1.705 5.951 Yes 10 mg GalNAc-ol acidic a-D-Neup5Ac-(2-6)-D-GalNAc-ol 636 (ID GlycomeDB 510) 199 spectrum_1D homonuclear Proton* 1D reduced Sialyl Tn [incr. 6] α-D-NeuAcp (2-6) (acidic) 200 rpalustris11 O-Glycan rpalustris11.jpg 879 902 11.224 15.602 Yes see barenarum7 GalNAc-ol 1 acidic b-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ 12269 (ID GlycomeDB 7771) 200 spectrum_1D homonuclear Proton* 1D Cad [incr. 3,3] α-D-NeuAcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-GalNAcp (1-4) (neutral) 201 rpalustris12 O-Glycan rpalustris12.jpg 822 845 11.538 15.921 Yes 1mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 9026 (ID GlycomeDB 6354) 201 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 202 rpalustris13 O-Glycan rpalustris13.jpg 879 902 11.073 15.465 Yes 0.1mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-GlcpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 202 spectrum_R1 homonuclear COSYR1* 2D [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GlcNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 203 rpalustris14 O-Glycan rpalustris14.jpg 879 902 11.413 15.805 No GalNAc-ol 1 acidic a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ no referenced 203 spectrum_R1 homonuclear COSYR1* 2D [incr. 3,3] α-D-NeuAcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GlcNAcp (1-4) (neutral) 204 rpalustris15 O-Glycan rpalustris15.jpg 1041 1064 15.819 20.230 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-3)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 204 spectrum_1D homonuclear Proton* 1D [incr. 3,3,4,3] α-D-NeuAcp (2-3) (acidic) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 205 rpalustris16 O-Glycan rpalustris16.jpg 1041 1064 15.667 20.056 Yes 2mg (see comments) GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 205 spectrum_R2 homonuclear COSYR2* 2D 205 spectrum_ROE homonuclear ROESY* 1D [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 206 rpalustris17 O-Glycan rpalustris17.jpg 1244 1267 20.674 25.063 Yes 2 mg (see comments) GalNAc-ol 1 acidic b-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ no referenced 206 spectrum_R2 homonuclear COSYR2* 2D 206 spectrum_ROE homonuclear ROESY* 2D Cad [incr. 3,3,4,3] α-D-NeuAcp (2-3) (acidic) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,4,3] β-D-GalNAcp (1-4) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 207 rpalustris18 O-Glycan rpalustris18.jpg 896 919 19.342 23.751 No GalNAc-ol 1 neutral a-L-Fucp-(1-2)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 207 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3,4,3] α-L-Fucp (1-2) (neutral) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 208 rpalustris19 O-Glycan rpalustris19.jpg 1115 1138 23.427 27.836 No Gal-ol 1 neutral b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced 208 spectrum_R2 homonuclear COSYR2* 2D 208 spectrum_ROE homonuclear ROESY* 2D [incr. 3,4,3,4,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 209 rpalustris20 O-Glycan rpalustris20.jpg 1480 1503 32.917 37.727 No GalNAc-ol 1 neutral b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol no referenced [incr. 3,4,3,4,3,4,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3,4,3] β-D-Galp (1-3) (neutral) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3,4,3,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 4,3,4,3] α-D-GalNAcp (1-4) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 210 rpalustris21 O-Glycan rpalustris21.jpg 676 699 6.165 10.543 No GalNAc-ol acidic Unpublished molecule in this paper, found in many species (bbufo1, barenarum5, bviridis10, xtropicalis10 and rpalustris21) Maes E., Florea D., Coppin A. and Strecvker G., 1999, Eur. J. Biochem, 264, 301-313 a-D-Neup5Ac-(2-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol 638 (ID GlycomeDB 512) 210 spectrum_1D homonuclear Proton* 1D reduced Sialyl T [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 211 rpalustris22 O-Glycan rpalustris22.jpg 676 699 6.345 10.733 Yes 2 mg (see comments) GalNAc-ol 1 acidic unpublished molecule, found (7%) in mixture with rpalustris10 (93%) a-D-Neup5Ac-(2-3)-b-D-Galp-(1-3)-D-GalNAc-ol 696 (ID GlycomeDB 553) 211 spectrum_1D homonuclear Proton* 1D [incr. 3,3] α-D-NeuAcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) 212 rpalustris23 O-Glycan rpalustris23.jpg 1041 4064 15.883 20.277 No GalNAc-ol 1 acidic unpublised molecule, in mixture with others, rpalustris16 and 17 b-D-Galp-(1-3)-a-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ no referenced 212 spectrum_1D homonuclear Proton* 1D [incr. 3,3] α-D-NeuAcp (2-3) (acidic) [incr. 3,4,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] α-D-GalNAcp (1-4) (neutral) 213 bbufo1 O-Glycan bbufo1.jpg 676 699 6.165 10.543 Yes 1.4 mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol 638 (ID GlycomeDB 512) 213 spectrum_90 homonuclear COSY90* 2D 213 spectrum_1D homonuclear Proton* 1D Sialyl T [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 214 bbufo2 O-Glycan bbufo2.jpg 692 715 6.186 10.566 Yes 5.3mg GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol 2108 214 spectrum_90 homonuclear COSY90* 2D 214 spectrum_1D homonuclear Proton* 1D Sialyl T [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 215 bbufo3 O-Glycan bbufo3.jpg 1025 1048 16.980 21.283 Yes 3.0 mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 215 spectrum_90 homonuclear COSY90* 1D 215 spectrum_1D homonuclear Proton* 1D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 216 bbufo4 O-Glycan bbufo4.jpg 1041 1064 17.001 21.297 Yes 1.4 mg GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 216 spectrum_90 homonuclear COSY90* 2D 216 spectrum_1D homonuclear Proton* 1D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 217 bbufo5 O-Glycan bbufo5.jpg 1187 1110 22.129 26.425 Yes 1.4 mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 217 spectrum_90 homonuclear COSY90* 2D 217 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 218 bbufo6 O-Glycan bbufo6.jpg 1203 1226 22.151 26.450 Yes 3mg GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 218 spectrum_90 homonuclear COSY90* 2D 218 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 219 bbufo7 O-Glycan bbufo7.jpg 1349 1372 27.244 31.504 No GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 219 spectrum_90 homonuclear COSY90* 2D 219 spectrum_R2 homonuclear COSYR2* 2D A Blood Group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 220 bbufo8 O-Glycan bbufo8.jpg 1203 1226 21.096 25.501 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol no 220 spectrum_90 homonuclear COSY90* 2D [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 221 bbufo9 O-Glycan bbufo9.jpg 1349 1372 26.488 30.898 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 221 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 222 bbufo10 O-Glycan bbufo10.jpg 1203 1226 21.375 25.780 No GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 222 spectrum_90 homonuclear COSY90* 2D A Blood Group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 223 bbufo11 O-Glycan bbufo11.jpg 1365 1388 26.512 30.917 Yes 2mg GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 223 spectrum_1D homonuclear Proton* 1D 223 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 224 bbufo12 O-Glycan spectrum_90 1511 1534 32.353 36.587 No GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 224 spectrum_90 homonuclear COSY90* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 225 bbufo14 O-Glycan bbufo14.jpg 1698 1721 36.919 41.152 No GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ b-D-GlcpNAc-(1-6)+ | | | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 225 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 226 bbufo15 O-Glycan bbufo15.jpg 1714 1737 36.924 41.159 Yes 3mg GalNAc-ol 1 acidic a-D-Neup5Gc-(2-6)+ b-D-GlcpNAc-(1-6)+ | | | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 226 spectrum_1D homonuclear Proton* 1D 226 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] α-D-NeuGcp (2-6) (acidic) 227 bbufo16 O-Glycan bbufo16.jpg 588 611 9.628 14.020 Yes 0.5mg GalNAc-ol 1 neutral unpublished for this species personal result a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol 684 227 spectrum_1D homonuclear Proton* 1D [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 228 bbufo17 O-Glycan bbufo17.jpg 734 757 15.290 19.590 Yes 1.5mg GalNAc-ol 1 neutral a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 639 (ID GlycomeDB 513) 228 spectrum_1D homonuclear Proton* 1D 228 spectrum_R2 homonuclear COSYR2* 2D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 229 bbufo18 O-Glycan bbufo18.jpg 937 960 19.833 24.133 Yes see barenarum12 GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 1674 (ID 1384 in GlycomeDB) 229 spectrum_90 homonuclear COSY90* 2D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 230 bbufo19 O-Glycan bbufo19.jpg 1302 1325 29.500 33.800 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-3)-b-D-GlcpNAc-(1-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 230 spectrum_R1 homonuclear COSY90* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,6] β-D-GlcNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 231 bbufo20 O-Glycan bbufo20.jpg 1204 1227 30.080 No Gal-ol neutral b-D-GlcpNAc-(1-6)+ a-L-Fucp-(1-2)+ | | | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-Gal-ol | a-L-Fucp-(1-2)+ no 231 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 232 bbufo21 O-Glycan bbufo21.jpg 1042 1065 25.537 29.787 Yes 0.2mg GalNAc-ol 1 neutral a-L-Fucp-(1-2)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 232 spectrum_90 homonuclear COSY90* 2D A Blood Group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 233 bbufo22 O-Glycan bbufo22.jpg 839 862 20.371 Yes 0.9mg Gal-ol neutral a-L-Fucp-(1-2)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-Gal-ol | a-L-Fucp-(1-2)+ no 233 spectrum_1D homonuclear Proton* 1D 233 spectrum_90 homonuclear COSY90* 2D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 234 bbufo23 O-Glycan bbufo23.jpg 693 716 15.402 Yes 1mg Gal-ol neutral a-L-Fucp-(1-2)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-D-Gal-ol no 234 spectrum_1D homonuclear Proton* 1D 234 spectrum_90 homonuclear COSY90* 2D [incr. 2] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-Galp (1-3) (neutral) [incr. 3] α-D-GalNAcp (1-3) (neutral) 235 bbufo24 O-Glycan bbufo24.jpg 896 919 20.431 24.731 Yes 2.5 mg GalNAc-ol 1 neutral a-L-Fucp-(1-2)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-GalNAc-ol no 235 spectrum_90 homonuclear COSY90* 2D 235 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 236 bbufo25 O-Glycan bbufo25.jpg 1099 1122 24.969 29.270 Yes 1.5 mg GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-GalNAc-ol | a-L-Fucp-(1-2)+ no 236 spectrum_R2 homonuclear COSYR2* 2D 236 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 237 bbufo26 O-Glycan bbufo26.jpg 1204 1227 No GalNAc-ol 1 neutral a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 237 spectrum_1D homonuclear Proton* 1D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 238 bbufo28 O-Glycan bbufo28.jpg 1407 1430 35.260 39.483 No GalNAc-ol 1 neutral b-D-GlcpNAc-(1-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 238 spectrum_R2 homonuclear COSYR2* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 239 bbufo29 O-Glycan bbufo29.jpg 1610 1633 39.792 44.002 No GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ b-D-GlcpNAc-(1-6)+ | | | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 239 spectrum_R1 homonuclear COSYR1* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 240 bbufo30 O-Glycan bbufo30.jpg 1772 1795 45.239 48.539 Yes 0.6 mg GalNAc-ol 2 neutral a-L-Fucp-(1-2)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-4)- b-D-GlcpNAc-(1-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 240 spectrum_R2 homonuclear COSYR2* 2D 240 spectrum_1D homonuclear Proton* 1D [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,6] α-L-Fucp (1-2) (neutral) [incr. 3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,4,6] α-D-Galp (1-3) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,4,6] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-Galp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 241 bbufo31 O-Glycan bbufo31.jpg 2080 2103 55.461 59.701 No GalNAc-ol 1 neutral a-L-Fucp-(1-2)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-4)- b-D-GlcpNAc-(1-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ no 241 spectrum_90 homonuclear COSY90* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2,4,6,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3,4,6,3] α-D-Galp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3,4,6,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6,3] β-D-Galp (1-4) (neutral) [incr. 6,3] β-D-GlcNAcp (1-6) (neutral) 242 bbufo32 O-Glycan bbufo32.jpg 1058 1081 24.775 29.191 GalNAc-ol 1 neutral a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ no 242 spectrum_90 homonuclear COSY90* 2D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 243 bbufo33 O-Glycan bbufo33.jpg 588 611 9.114 13.402 Yes 5 mg GalNAc-ol 3 neutral unpublished input by Jorick Vanbeselaere b-D-Galp-(1-3)-b-D-GlcpNAc-(1-3)-D-GalNAc-ol 3668 243 spectrum_1D homonuclear Proton* 1D [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-GlcNAcp (1-3) (neutral) 244 bbufo35 O-Glycan bbufo35.jpg 1495 1518 32.341 Yes 5.3 mg GalNAc-ol 1 acidic Isomers of bbufo12, NeuAc instead of NeuGc input by Jorick Vanbeselaere a-D-Neup5Ac-(2-6)+ | a-D-Galp-(1-3)-a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | | a-L-Fucp-(1-2)+ a-L-Fucp-(1-2)+ 244 spectrum_1D homonuclear Proton* 1D [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3,3,3] α-D-Galp (1-3) (neutral) [incr. 3,3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 245 barenarum1 O-Glycan barenarum1.jpg 531 554 9.835 14.231 Yes 6 mg GalNAc-ol 1 neutral a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 80 (ID GlycomeDB 69) 245 spectrum_1D homonuclear Proton* 1D 245 spectrum_90 homonuclear COSY90* 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 246 barenarum2 O-Glycan barenarum2.jpg 636 659 15.194 Yes 4 mg (in mixture) Gal-ol neutral a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-Gal-ol | a-L-Fucp-(1-2)+ no 246 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 2] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 247 barenarum3 O-Glycan barenarum3.jpg 734 757 14.348 18.750 Yes 4 mg (in mixture) GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 637 (ID GlycomeDB 511) 247 spectrum_1D homonuclear Proton* 1D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 248 barenarum4 O-Glycan barenarum4.jpg 734 757 15.290 19.590 Yes 4 mg (in mixture) GalNAc-ol 1 neutral a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 639 (ID GlycomeDB 513) 248 spectrum_1D homonuclear Proton* 1D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) 249 barenarum6 O-Glycan barenarum6.jpg 822 845 11.538 15.921 Yes 28 mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-D-GalNAc-ol 9026 (ID GlycomeDB 6354) 249 spectrum_1D homonuclear Proton* 1D 249 spectrum_90 homonuclear COSY90* 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 250 barenarum7 O-Glycan barenarum7.jpg 879 902 11.224 15.602 Yes 2.5 mg GalNAc-ol 1 acidic b-D-GalpNAc-(1-4)-b-D-Galp-(1-3)-D-GalNAc-ol | a-D-Neup5Ac-(2-3)+ 12269 (ID GlycomeDB 7771) 250 spectrum_1D homonuclear Proton* 1D 250 spectrum_90 homonuclear COSY90* 2D Cad [incr. 3,3] α-D-NeuAcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,3] β-D-GalNAcp (1-4) (neutral) 251 barenarum8 O-Glycan barenarum8.jpg 1025 1048 16.980 21.238 Yes 0.2mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 251 spectrum_1D homonuclear Proton* 1D 251 spectrum_90 homonuclear COSY90* 2D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 252 barenarum9 O-Glycan barenarum9.jpg 1130 1153 22.005 26.250 Yes 2.6 mg GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)-b-D-Galp-(1-3)+ | a-L-Fucp-(1-2)+ no referenced 252 spectrum_1D homonuclear Proton* 1D 252 spectrum_90 homonuclear COSY90* 2D H group [incr. 2,3,3] α-L-Fucp (1-2) (neutral) [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] β-D-Galp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 253 barenarum10 O-Glycan barenarum10.jpg 814 837 14.402 18.795 Yes 0.7 mg GalNAc-ol 2 acidic Input with helping of Cindy Dieryckx. Corresponding HPLC peak : 200-I-5 in paper (Morelle W., Cabada O and Strecker G., 1998, Eur. J. Biochem., 252, 253-260). Molecule is identical with rdalmatina12 and rtemporaria16. HSO3(6)-b-D-GlcpNAc-(1-6)-D-GalNAc-ol | a-L-Fucp-(1-2)-b-D-Galp-(1-3)+ no referenced 253 spectrum_1D homonuclear Proton* 1D 253 spectrum_90 homonuclear COSY90* 2D H group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (acidic) with sulfate (6) 254 barenarum11 O-Glycan barenarum11.jpg 223 246 4.252 Yes 2.3 mg GalNAc-ol neutral D-GalNAc-ol 1561 (ID GlycomeDB 1276) 254 spectrum_1D homonuclear Proton* 1D 255 barenarum12 O-Glycan barenarum12.jpg 937 960 19.833 24.133 Yes 0.9 mg GalNAc-ol 2 neutral b-D-GlcpNAc-(1-6)+ | a-D-GalpNAc-(1-3)-b-D-Galp-(1-3)-D-GalNAc-ol | a-L-Fucp-(1-2)+ 1674 (ID 1384 in GlycomeDB) 255 spectrum_1D homonuclear Proton* 1D 255 spectrum_90 homonuclear COSY90* 2D A Blood Group [incr. 2,3] α-L-Fucp (1-2) (neutral) [incr. 3,3] α-D-GalNAcp (1-3) (neutral) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral) 256 barenarum13 O-Glycan barenarum13.jpg 439 462 1.703 5.946 Yes 0.1 mg Gal-ol acidic a-D-Neup5Ac-(2-6)-Hexen-tetrol no referenced 256 spectrum_1D homonuclear Proton* 1D 256 spectrum_90 homonuclear COSY90* 2D [incr. 6] α-D-NeuAcp (2-6) (acidic) 257 barenarum5 O-Glycan barenarum5.jpg 676 699 6.165 10.543 Yes GalNAc-ol 1 acidic a-D-Neup5Ac-(2-6)+ | b-D-Galp-(1-3)-D-GalNAc-ol 638 (ID GlycomeDB 512) 257 spectrum_1D homonuclear Proton* 1D reduced Sialyl T [incr. 3] β-D-Galp (1-3) (neutral) [incr. 6] α-D-NeuAcp (2-6) (acidic) 258 talpestris27 O-Glycan talpestris27.jpg 1082 1105 15.379 19.764 No GalNAc-ol 2 acidic Molecule is in mixture with talpestris30. It corresponds to IV-5 and III-2 HPLC peaks. (b5 for us) b-D-GalpNAc-(1-4)-b-D-GlcpNAc-(1-6)+ | a-D-Neup5Ac-(2-3)-b-D-Galp-(1-3)-D-GalNAc-ol no 258 spectrum_1D homonuclear Proton 1D 258 spectrum_90 homonuclear COSY90 2D 258 spectrum_R1 homonuclear COSYR1* 2D 258 spectrum_R2 homonuclear COSYR2 2D 258 spectrum_ROE homonuclear ROESY 2D LacDiNAc [incr. 2,3] α-D-NeuAcp (2-3) (acidic) [incr. 3] β-D-Galp (1-3) (neutral) [incr. 4,6] β-D-GalNAcp (1-4) (neutral) [incr. 6] β-D-GlcNAcp (1-6) (neutral)